تأثیر ژنوتیپ و محیط کشت کالوس‌زایی بر کارایی کشت بساک هیبریدهای برنج ایندیکا × ایندیکای هتروتیک

This study was set up in the research station of Rice Research Institute of Iran (RRII) to determine the response of three hetrotic Indica × Indica rice hybrids (Bahar1, IR75221H and IR69688H) to anther culture on four induction media (N6, modified N6, chu and modified chu). To evaluate hybrids, the percentage of calluses which had been made from anthers in early uni- to mid- uninucleate stage and the number of regenerated plantlets of calluses in those media were determined. The results indicated that genotype, composition of callus induction medium and their interactions significantly affected callus percentage, total plant regeneration and production of green and albino plants. IR75221H hybrid was considerably superior for callus induction (1/64%) and Bahar1 hybrid was the most desirable for producing of green plants (3/43%) and total regeneration (12/24%). IR75221H hybrid produced higher albino plant (10/66%) and IR69688H hybrid produced lower albino plant (6/45%). The best medium culture in the case of callus induction was modified N6 (2%) and total regeneration (21/82%) produced green plant (5/6%), and albino plant (16/22%) was modified Chu among all media culture. However, genotypes showed different results in each media culture, and responses to hybrid of anther culture was low, but by changing the media culture we increased the response of culture.